Transmission-blocking antibodies against mosquito C-type lectins for dengue prevention.

Transmission-blocking antibodies against mosquito C-type lectins for dengue prevention.

Blog Article

C-type lectins are a family of proteins with carbohydrate-binding activity.Several C-type lectins in mammals or arthropods are employed as receptors or attachment factors to facilitate flavivirus invasion.We previously identified a C-type lectin in Aedes aegypti, designated as mosquito galactose shiro neon zero specific C-type lectin-1 (mosGCTL-1), facilitating the attachment of West Nile virus (WNV) on the cell membrane.Here, we first identified that 9 A.

aegypti mosGCTL genes were key susceptibility factors facilitating DENV-2 infection, of which mosGCTL-3 exhibited the most significant effect.We found that mosGCTL-3 was induced in mosquito tissues with DENV-2 infection, and that the protein interacted with DENV-2 surface envelop (E) protein and virions in vitro and in vivo.In addition, the other identified mosGCTLs interacted with the DENV-2 E protein, indicating that DENV case of whispering angel may employ multiple mosGCTLs as ligands to promote the infection of vectors.The vectorial susceptibility factors that facilitate pathogen invasion may potentially be explored as a target to disrupt the acquisition of microbes from the vertebrate host.

Indeed, membrane blood feeding of antisera against mosGCTLs dramatically reduced mosquito infective ratio.Hence, the immunization against mosGCTLs is a feasible approach for preventing dengue infection.Our study provides a future avenue for developing a transmission-blocking vaccine that interrupts the life cycle of dengue virus and reduces disease burden.